Caenorhabditis elegans strains were maintained on NGM plates and OP50 Escherichia coli bacteria at 20°C as described (Brenner, 1974)31 (link), except that daf-2 mutants (and corresponding controls for a given assay) were maintained at 15°C unless otherwise noted. The wild-type strain was N2 Bristol31 (link). Mutant strains used are described in Wormbase (www.wormbase.org ): LGI: daf-16(mgDf47, mu86); LGII: eat-2(ad1116); LGIII: daf-2(e1368, e1370, and m596), rrf-3(pk1462), glp-1(bn18); and LGIV: eri-1(mg366), skn-1(tm3411, zu67, zu129, and zu135). LGX: lin-15B(n744). Transgenic lines used were: jgIs5 [ROL- 6::GFP;TTX-3::GFP]32 (link), BC12533 dpy-5(e907); sEx12533 [Pcol-89::GFP; dpy-5(+)]33 (link), CF1660 daf-16(mu86); daf-2(e1370); muIs84 [Psod-3::GFP; pRF4 rol-6(su1006gf)]; muEx211 [Pges-1::DAF-16::GFP; pRF4 rol-6(su1006gf)]15 (link), CL2166 dvIs19 [Pgst-4::GFP; pRF4 rol-6(su1006gf)]34 (link), EE86 mup-4(mg36); upIs1 [MUP-4::GFP; pRF4 rol-6(su1006gf)]35 (link), HT1883 daf-16(mgDf50); daf-2(e1370) unc-119(ed3); lpIs14 [Pdaf-16::DAF-16f::GFP + unc-119(+)]36 (link), IG274 frIs7 [Pcol-12::DsRed; Pnlp-29::GFP]37 (link), LD001 ldIs007 [Pskn-1::SKN-1b/c::GFP; pRF4 rol-6(su1006gf)]38 (link), MH2051 kuIs55 [LON-3::GFP; unc-119(+)]39 (link), SJ4005 zcIs4 [Phsp-4::GFP; lin-15(+)]40 (link), SJ4103 zcIs14 [myo-3::GFP(mit)]41 (link), TB1682 chEx1682 [QUA-1::GFP; pRF4 rol-6(su1006gf)]42 (link), TJ356 zIs356 [Pdaf-16::DAF-16a/b::GFP; pRF4 rol-6(su1006gf)]43 (link), TP12 kaIs12 [COL-19::GFP]44 (link).
Protocol detail
Caenorhabditis elegans Strain Maintenance
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Assay
Bacteria
Caenorhabditis elegans
Eat 2
Escherichia
Escherichia coli
Glp 1
Pges 1
Strains
Transgenic
Corresponding Organization : Princeton University
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Variable analysis
independent variables
- Caenorhabditis elegans strains
- Not explicitly mentioned
- Temperature (20°C for wild-type strains, 15°C for daf-2 mutants)
- NGM plates and OP50 Escherichia coli bacteria as food source
- Corresponding controls for a given assay for daf-2 mutants
- Wild-type N2 Bristol strain
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