Levonorgestrel Downregulation and Losartan Extinction

Levonorgestrel (Sigma-Aldrich), a progestin-only HC shown to downregulate estradiol levels^{3 (link)}, was dissolved 1:1 in double distilled water and dimethyl sulfoxide (DMSO; Fisher Scientific) and administered subcutaneously (s.c.) at a dose of 0.5 mg/kg/day 4 days prior to and throughout the fear conditioning protocol^{3 (link)}. Losartan potassium (Sigma-Aldrich), an AT1R antagonist, was dissolved in sterile saline and injected intraperitoneally (i.p.) at doses of 3 mg/kg or 10 mg/kg approximately 5 min before fear extinction^{8 (link)}.

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Parrish J.N., Bertholomey M.L., Pang H.W., Speth R.C, & Torregrossa M.M. (2019). Estradiol modulation of the renin–angiotensin system and the regulation of fear extinction. Translational Psychiatry, 9, 36.

Publication 2019

Levonorgestrel DMSO Losartan potassium

Conditioning fear Dmso Estradiol Fear Levonorgestrel Losartan potassium Progestin Saline Sterile

Corresponding Organization :

Other organizations : University of Pittsburgh, Nova Southeastern University

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Variable analysis

independent variables

Levonorgestrel (Sigma-Aldrich), a progestin-only HC, administered subcutaneously (s.c.) at a dose of 0.5 mg/kg/day 4 days prior to and throughout the fear conditioning protocol
Losartan potassium (Sigma-Aldrich), an AT1R antagonist, injected intraperitoneally (i.p.) at doses of 3 mg/kg or 10 mg/kg approximately 5 min before fear extinction

dependent variables

Not explicitly mentioned

control variables

Double distilled water and dimethyl sulfoxide (DMSO; Fisher Scientific) used to dissolve Levonorgestrel
Sterile saline used to dissolve Losartan potassium

positive controls

None specified

negative controls

None specified

Annotations

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