Maintaining Human Pluripotent Stem Cells

HESC lines HES3 and HUES8 and the hiPSC line hCBiPSC2^{51 (link)} were cultivated as described earlier^{4 (link),27 ,30 (link)}. Briefly, all hPSCs were cultured as colonies on cell culture plastic coated with hESC-qualified Matrigel (Corning, Amsterdam, Netherlands). As cultivation medium mTeSR1 (Stem Cell Technologies, Cologne, Germany) or StemMACS™ iPS-Brew XF (Miltenyi Biotec, Bergisch Gladbach, Germany) was used. Passaging was performed every 5–7 days using a non-enzymatic solution^{52 (link)} or Dispase (5 U/mL) in a ratio of 1:6 up to 1:40 onto Matrigel-coated 6-well plates.

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Diekmann U., Wolling H., Dettmer R., Niwolik I., Naujok O, & Buettner F.F. (2019). Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture. Scientific Reports, 9, 996.

Publication 2019

Matrigel mTeSR1 StemMACS™ iPS-Brew XF

Cell culture Dispase Enzymatic Hesc Hipsc Matrigel Stem cell

Corresponding Organization :

Other organizations : Medizinische Hochschule Hannover

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Variable analysis

independent variables

Cell lines: HESC lines HES3 and HUES8, and hiPSC line hCBiPSC2

dependent variables

Not explicitly mentioned

control variables

Cultivation of all hPSCs on cell culture plastic coated with hESC-qualified Matrigel
Use of mTeSR1 or StemMACS™ iPS-Brew XF as cultivation medium
Passaging performed every 5–7 days using a non-enzymatic solution or Dispase (5 U/mL) in a ratio of 1:6 up to 1:40 onto Matrigel-coated 6-well plates

controls

Positive control: Not specified
Negative control: Not specified

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