The experiment was described previously [36 (link),77 (link)]. In short, peroxidation of PC liposome lipids was initiated with ultraviolet radiation using a bactericidal UVB lamp. Peroxidation was measured as the TBARS level. The percentage of PC liposome oxidation inhibition was calculated using the formula:
where CC refers to the concentration of malondialdehyde (MDA) in a sample without extract (control) and CE refers to the concentration of MDA in a sample with the appropriate extract added, measured at λ = 535 nm. All determinations were performed with three independent preparations (n = 3) using a Cary 300 Varian spectrophotometer.
where CC refers to the concentration of malondialdehyde (MDA) in a sample without extract (control) and CE refers to the concentration of MDA in a sample with the appropriate extract added, measured at λ = 535 nm. All determinations were performed with three independent preparations (n = 3) using a Cary 300 Varian spectrophotometer.
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