Immunocytochemistry for Breast Cancer Markers

Immunocytochemistry (IHC) was performed using ER (1D5; Dako, Tokyo, Japan) and PgR (PgR636; Dako), Ki-67(MIB-1; Dako), PTEN (6H2.1; Dako) antibodies and HercepTest II (Dako) following the manufacturer’s instructions. The stained slides were reviewed by two observers without knowledge of the clinical data. Staining as scored semi quantitatively using a histoscore (H-score), which was calculated as staining intensity (scored as 0–3) x percentage of stained cells (0–100%) as previously described [19 (link)]. Because the scoring system for PTEN expression by using immunohistochemical methods are not standardized, PTEN H-scores less than 50 were considered as low in this analysis,[20 (link)].

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Hayama S., Nakamura R., Ishige T., Sangai T., Sakakibara M., Fujimoto H., Ishigami E., Masuda T., Nakagawa A., Teranaka R., Ota S., Itoga S., Yamamoto N., Nagashima T, & Otsuka M. (2023). The impact of PIK3CA mutations and PTEN expression on the effect of neoadjuvant therapy for postmenopausal luminal breast cancer patients. BMC Cancer, 23, 384.

Publication 2023

ER (1D5; Ki-67(MIB-1; HercepTest II

Antibodies Cells Immunocytochemistry Mib 1 Pten

Corresponding Organization : Kitasato University

Other organizations : Chiba University, Chiba Cancer Center, Toho University Medical Center Sakura Hospital, Toho University, Chiba University Hospital, Kazusa DNA Research Institute

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Variable analysis

independent variables

ER (1D5; Dako, Tokyo, Japan)
PgR (PgR636; Dako)
Ki-67(MIB-1; Dako)
PTEN (6H2.1; Dako)
HercepTest II (Dako)

dependent variables

Staining intensity (scored as 0–3)
Percentage of stained cells (0–100%)
PTEN H-scores (less than 50 considered as low)

control variables

The stained slides were reviewed by two observers without knowledge of the clinical data.

controls

No positive or negative controls were explicitly mentioned.

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