Detailed Cellular Assays for Cancer Research

Assays for Western blot, cell viability by MTS(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, Inner Salt), anchorage-independent growth, and invasion were performed as described previously [47 (link),48 (link)]. Detailed descriptions are accessible in the Supplementary Materials and Methods. The following antibodies were used in Western blot: anti-SOX1 (R&D Systems, Minneapolis, MN, USA), anti-HES1 (Cell Signal, Danvers, MA, USA).

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Chang S.Y., Wu T.H., Shih Y.L., Chen Y.C., Su H.Y., Chian C.F, & Lin Y.W. (2023). SOX1 Functions as a Tumor Suppressor by Repressing HES1 in Lung Cancer. Cancers, 15(8), 2207.

Publication 2023

anti-SOX1 anti-HES1

Antibodies Cell Cell viability Salt Tetrazolium Western blot Western blot assays

Corresponding Organization : National Defense Medical Center

Other organizations : Tri-Service General Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Western blot
Cell viability by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, Inner Salt)
Anchorage-independent growth
Invasion

control variables

None explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

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