Example 17
Mice imaging was approved by the Institutional Animal Care and Use Committee (IACUC) of the Children's Hospital of Los Angeles (permit number: 38616) and of the University of Southern California (permit number: 20685). Experimental research on vertebrates complied with institutional, national and international ethical guidelines. Animals were kept on a 13:11 hours light:dark cycle. Animals were breathing double filtered air, temperature in the room was kept at 68-73 F, and cage bedding was changed weekly. All these factors contributed to minimize intra- and inter-experiment variability. Adult 8 weeks old C57Bl mice were euthanized with euthasol. Tracheas were quickly harvested from the mouse, washed in PBS, and cut longitudinally alongside the muscolaris mucosae in order to expose the lumen. A 3 mm×3 mm piece of the trachea was excised and arranged onto a microscope slide for imaging.
