Two slides from patient UM-001 were stained with pan-cytokeratin (CK) and CD45 antibodies as described above with DAPI24 (link) complemented with a monoclonal EpCAM antibody (1:250, 324202, Biolegend, San Diego, CA; Alexa Fluor® 488 goat anti-mouse IgG2b secondary antibody, 1:500, A21141, Invitrogen, Carlsbad, CA) at Epic Sciences. CTCs were identified with standard image analysis protocols25 (link)–35 (link).
Free full text: Click here