Comprehensive Western Blot Methodology

Western blot was performed as previously described^{20 (link)}. Antibodies used in this study were PDLIM1 (Affinity, DF3003), α-SMA (Affinity, AF1032), COL1A1 (Cell Signaling Technology, #91144), CTCF (Millipore, 07-729), TNF-α (Affinity, AF7014), IL-6 (Affinity, DF6087), p65(Affinity, AF5006), anti-p-Smad2/3 (Abcam, ab254407), anti-Smad2/3 (Abcam, ab202445), anti-p-ERK (Abcam, ab201015), anti-ERK (Abcam, ab184699), anti-p-P38 (Proteintech, 28796-1-AP), anti-P38 (Proteintech, 66234-1-Ig), anti-p-JNK (Proteintech, 80024-1-RR), anti-JNK (Proteintech, 66210-1-Ig), GAPDH (Cell Signaling Technology, #2118). Western blot analysis was performed on at least three independent biological replicates.

Free full text: Click here

Ye B., Yu M., Yue M., Yin M., Zhang C., Wang Q., Ding X., Shen W, & Zhao Z. (2023). Role of PDLIM1 in hepatic stellate cell activation and liver fibrosis progression. Scientific Reports, 13, 10946.

Publication 2023

α-SMA COL1A1 TNF-α IL-6 ab254407 ab202445 anti-p-ERK ab201015 anti-ERK anti-p-P38 anti-P38 anti-p-JNK anti-JNK GAPDH

Antibodies Biological Ctcf Gapdh Smad2 Tnf α Western blot Western blot analysis

Corresponding Organization : Henan Normal University

Other organizations : Chinese Academy of Sciences, Institute of Genetics and Developmental Biology, Beijing Fengtai Hospital

Top 5 similar protocols

Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Protein expression levels of PDLIM1, α-SMA, COL1A1, CTCF, TNF-α, IL-6, p65, p-Smad2/3, Smad2/3, p-ERK, ERK, p-P38, P38, p-JNK, JNK, and GAPDH

control variables

Not explicitly mentioned

controls

Western blot analysis was performed on at least three independent biological replicates, which can be considered a form of experimental control.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!