Pancreatic cancer cell-line PANC-1 was procured from ATCC (CRL-1469; Manassas, VA, USA) and cultured in Dulbecco’s Modified Eagle’s Medium (30–2002, ATCC) supplemented with 10% FBS (Sigma, St.Louis, MI, USA). MIA PaCa-2 was obtained from ATCC (CRM-CRL-1420) and cultured in Dulbecco’s Modified Eagle’s Medium (30–2002, ATCC) supplemented with 10% FBS. AsPC-1 was obtained from ATCC (CRL-1682), and cultured in an RPMI-1640 medium (30–2001, ATCC) supplemented with 10% FBS (Sigma). Capan-1 was obtained from ATCC (HTB-79), and cultured in an Iscove’s Modified Dulbecco’s Medium (30–2005, ATCC) supplemented with 20% FBS (Sigma).
A human pancreatic nestin-expressing cell line,27 (link) hTERT-HPNE, was obtained from ATCC and cultured in amixture of 75% DMEM without glucose (Cat#. D-5030; Sigma, with additional 2 mM L-glutamine and 1.5 g/L sodium bicarbonate) and 25% Medium M3 Base (Cat# M300F-500; Incell Corp., San Antonio, TX, USA) supplemented with 10% FBS (Sigma). All the cells were cultured at 37°C in 5% CO2.
A human pancreatic nestin-expressing cell line,27 (link) hTERT-HPNE, was obtained from ATCC and cultured in amixture of 75% DMEM without glucose (Cat#. D-5030; Sigma, with additional 2 mM L-glutamine and 1.5 g/L sodium bicarbonate) and 25% Medium M3 Base (Cat# M300F-500; Incell Corp., San Antonio, TX, USA) supplemented with 10% FBS (Sigma). All the cells were cultured at 37°C in 5% CO2.
Free full text: Click here
