Women had an obstetrical, hematologic, and biochemical evaluation at enrollment. HIV-RNA VL (Abbott m2000 RealTime© HIV-1 assay; Abbott Molecular Inc., Des Plaines, IL; limit of quantification 40 copies/mL) and CD4 cell count were measured at baseline and delivery.
Infants were examined at birth and 2 weeks, 1, 2, 4, and 6 months of life. The child’s interval history was recorded, a physical examination performed, and blood obtained for HIV-DNA testing by real-time polymerase chain reaction (PCR) assay on peripheral blood spotted onto filter papers, dried, and stored at −20°C.13 (link) Hematology and chemistry tests were performed soon after birth, at 2 and 4 weeks.
Infants were examined at birth and 2 weeks, 1, 2, 4, and 6 months of life. The child’s interval history was recorded, a physical examination performed, and blood obtained for HIV-DNA testing by real-time polymerase chain reaction (PCR) assay on peripheral blood spotted onto filter papers, dried, and stored at −20°C.13 (link) Hematology and chemistry tests were performed soon after birth, at 2 and 4 weeks.
