Illumina TruSeq PCR-free Sequencing of Canine Genome

An Illumina TruSeq PCR-free library with ~400 bp insert size of an affected dog from family 1 was prepared. We collected 221 million 2 × 150 bp paired-end reads on a NovaSeq 6000 instrument (35.5 × coverage). Mapping and alignment were performed as described in [15 (link)]. The sequence data were deposited under study accession PRJEB16012 and sample accession SAMEA112638879 at the European Nucleotide Archive.

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Christen M., Zdora I., Leschnik M., Jagannathan V., Puff C., Hünerfauth E., Volk H.A., Baumgärtner W., Koch T.C., Schäfer W., Kleiter M, & Leeb T. (2023). RALGAPA1 Deletion in Belgian Shepherd Dogs with Cerebellar Ataxia. Genes, 14(8), 1520.

Publication 2023

TruSeq PCR

Bp 400 European Library Nucleotide

Corresponding Organization : University of Bern

Other organizations : University of Veterinary Medicine Hannover, Foundation, University of Veterinary Medicine Vienna

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Variable analysis

independent variables

Preparation of an Illumina TruSeq PCR-free library with ~400 bp insert size from an affected dog from family 1

dependent variables

221 million 2 × 150 bp paired-end reads collected on a NovaSeq 6000 instrument (35.5 × coverage)

control variables

Mapping and alignment as described in [15 (link)]

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