Mitochondrial membrane potential was measured in cells after treatment with varying concentrations of glucose/palmitic acid for 24 h using a fluorescent cationic dye, DePsipher TM (R&D System Inc.), as described previously [25 (link),26 (link),27 (link)]. If the membrane potential is reduced, the dye cannot access the transmembranee space and remains in its green fluorescent form.
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