iPS Cell Culture from Blood Monocytes

The iPS cells derived from peripheral blood monocytes of male AD patients (66540594-1VL), and normal healthy control (66540083-1VL) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Cell culture was performed in reference to a reported method [21 (link)]. The iPS cells were cultured in mTesR1 complete medium (STEM CELL Technologies, Vancouver, BC, Canada) diluted with BD Matrigel (BD Biosciences, USA). The culture medium was changed every day. Cells were passaged every 4 days by using 0.5 mM ethylenediaminetetraacetic acid (EDTA) digestion for 3 min. Cells were maintained in a 5% CO₂ incubator at 37 °C.