Purification and Characterization of Mptx2 Antibody

The protein was purified using a nickel–nitrilotriacetic acid (Ni-NTA) column, a PD MidiTrap G-25 column (GE Healthcare, Chicago, IL, USA), and a Vivaspin 20 centrifugal concentrator (GE Healthcare) according to a published protocol^{18 (link)}. The purified protein was assessed using Coomassie Brilliant Blue staining. We purified the antibody against Mptx2 via antigen immunoaffinity. Reactivity was assessed using an enzyme-linked immunosorbent assay (ELISA).

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Yan W., Chen S., Wang Y., You Y., Lu Y., Wang W., Wu B., Du J., Peng S., Cai W, & Xiao Y. (2024). Loss of Mptx2 alters bacteria composition and intestinal homeostasis potentially by impairing autophagy. Communications Biology, 7, 94.

Publication 2024

PD MidiTrap G-25 column Vivaspin 20 centrifugal concentrator

Corresponding Organization :

Other organizations : XinHua Hospital, Shanghai Jiao Tong University, Shanghai Institute of Nutrition and Health

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Variable analysis

independent variables

Purification of the protein using a nickel–nitrilotriacetic acid (Ni-NTA) column
Purification of the protein using a PD MidiTrap G-25 column (GE Healthcare, Chicago, IL, USA)
Purification of the protein using a Vivaspin 20 centrifugal concentrator (GE Healthcare)
Purification of the antibody against Mptx2 via antigen immunoaffinity

dependent variables

Purity of the purified protein assessed using Coomassie Brilliant Blue staining
Reactivity of the purified antibody against Mptx2 assessed using an enzyme-linked immunosorbent assay (ELISA)

control variables

Published protocol used for purification of the protein (link)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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