Sedentary behavior will be assessed by three indicators including total daily sedentary time, prolonged sedentary time (time spent sitting >30 minutes and >60 minutes), and number of sit-to-stand transitions. These three sedentary behaviors will be measured by 7 days of activPAL 3 (PAL Technologies, U.K) device monitoring prior to the beginning of the intervention/control protocol and again after the conclusion of the protocol for both study arms. The activPAL 3 monitor is a tri-axial accelerometer that also includes an inclinometer, worn on thigh (Fig 4). Due to the activPAL 3 position on the thigh, the activPAL 3 monitor is the most sensitive device to distinguish sitting (horizontal position) and standing (vertical position) [43 (link), 44 (link)]. The activPAL 3 has demonstrated great test-retest reliability and criterion validity with direct observation to classify sitting, standing, and stepping [45 (link)].
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