LKBtide, Cat# 12-540, donepezil hydrochloride, ≥98%, Cat# D6281; phenformin hydrochloride, Cat# P7045; and berberine chloride, Cat# B3251, were from Sigma-Aldrich; p-nitrophenyl phosphate was from New England Biolabs, Ipswich, MA, USA, Cat# PO757S. BAY-3827 and BAY974 were from the Structural Genomics Consortium, Toronto, ON, Canada, (donated chemical probes); MK-8722 was synthesized in-house, as in [50 ]. SBI-0206965 was from Selleckchem, Houston, TX, USA, Cat# S7885. Acetonitrile (LC:MS grade), Cat# A19862.AP and formic acid (LC:MS grade), Cat# 270480250) were from ThermoFisher Scientific, Waltham, MA, USA. AMPK was purified from rat liver, as in [8 (link)]. Human (His)6-tagged AMPK (α1β2γ1, bacterially expressed, generated as in [51 (link)], was a gift from Dr Mark Peggie, University of Dundee, Dundee, UK; human (His)6-tagged AMPK (α2β2γ1, bacterially expressed) generated as in [51 (link)] was a gift from AstraZeneca, Cambridge, UK; human GST-tagged α2 kinase domain (1–310) was produced as described [44 (link)]; and human LKB1:STRAD-α:MO25-α complex [52 (link)], GST-tagged CaMKK2 [53 (link)], and PP2Cα (PPM1A) [54 (link)] were produced as described.
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