64Cu-CANF-comb and comb nanoparticles were reconstituted in 0.9% sodium chloride (APP pharmaceuticals) for intravenous (i.v.) injection. Wild-type C57BL/6 mice weighing 25–32 g were anesthetized with inhaled isoflurane and injected with approximately 370 kBq of the radiolabeled nanoparticles (~ 4.0 μg/kg body weight) in 100 μL saline via tail vein. The mice were re-anesthetized before euthanizing by cervical dislocation at each time point (1 h, 4 h, and 24 h, n = 4/time point) post injection (p.i.). Organs of interest were collected, weighed and counted in a well gamma counter (Beckman 8000, San Diego, CA). Standards were prepared and measured along with the samples to calculate the percentage of the injected dose per gram of tissue (% ID/gram) or percentage of the injected dose per organ of tissue (%ID/organ) (35 (link)).
Protocol detail
Biodistribution of Radiolabeled Nanoparticles in Mice
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Body weight
C57bl mice
Cervical
Comb
Dislocation
Gamma
Isoflurane
Mice
Pharmaceuticals
Saline
Tail
Tissue
Vein
Corresponding Organization :
Other organizations : Washington University in St. Louis, University of California, Santa Barbara
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Variable analysis
independent variables
- Injection of 64Cu-CANF-comb and comb nanoparticles
- Percentage of the injected dose per gram of tissue (% ID/gram)
- Percentage of the injected dose per organ of tissue (%ID/organ)
- Mouse strain (Wild-type C57BL/6)
- Mouse weight (25–32 g)
- Anesthesia (Inhaled isoflurane)
- Injection volume (100 μL saline)
- Injection route (Intravenous, via tail vein)
- Euthanasia method (Cervical dislocation)
- Time points (1 h, 4 h, and 24 h post-injection)
- Positive control: Standards prepared and measured along with the samples
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