Immunohistochemical Staining of TMA

Tumor tissue microarrays (TMA) blocks were prepared, cut and stained as previously described.
²² (link) Once diagnosis confirmed by an experienced pathologist, immunohistochemical staining were performed on TMA slides as previously described.
²² (link) TMA slides were incubated with E2F1 (VD301790, Thermo Fischer, 1:100 diluted in Dako REAL Antibody Diluent S2022) or E2F8 antibody (ab185727, Abcam, 1:500 diluted in Dako REAL Antibody Diluent S2022) overnight or for 60 min, respectively. Brain and thyroid tissue served as positive control.

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Bertonnier‐Brouty L., Andersson J., Kaprio T., Hagström J., Bsharat S., Asplund O., Hatem G., Haglund C., Seppänen H., Prasad R.B, & Artner I. (2024). E2F transcription factors promote tumorigenicity in pancreatic ductal adenocarcinoma. Cancer Medicine, 13(9), e7187.

Publication 2024

Antibody Diluent S2022

Corresponding Organization : Lund University

Other organizations : University of Helsinki, Helsinki University Hospital, University of Turku

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Variable analysis

independent variables

Incubation time of TMA slides with E2F1 antibody (overnight)
Incubation time of TMA slides with E2F8 antibody (60 min)

dependent variables

Immunohistochemical staining of TMA slides with E2F1 and E2F8 antibodies

control variables

TMA block preparation, cutting, and staining as previously described
Diagnosis confirmation by an experienced pathologist
Antibody dilutions (E2F1 at 1:100, E2F8 at 1:500)
Antibody diluent (Dako REAL Antibody Diluent S2022)
Brain and thyroid tissue as positive controls

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