Quantifying Vascular Density in Tissue Sections

The sections were stained with hematoxylineosin or prepared for immunohistochemical/fluorescence staining. Monoclonal antibodies specific for von Willbrand factor (vWF; Dako), tropomyosin (Santa Cruz Biotechnology), and a-sarcomeric actinin (Sigma) in pigs were used for immunohistochemical staining. The vWF-positive capillaries were counted under 200× microscopy. Twenty fields were randomly selected in each patch. Capillary density was expressed as the mean number of vessels per square millimeter.^{4 (link)}

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Koda Y., Watanabe T., Kawaji K., Mo F., Beaser A.D., Vaicik M., Hibino N, & Ota T. (2023). In Situ Myocardial Regeneration With Tissue Engineered Cardiac Patch Using Spheroid-Based 3-Dimensional Tissue. Annals of thoracic surgery short reports, 2(1), 150-155.

Publication 2023

tropomyosin a-sarcomeric actinin

Corresponding Organization :

Other organizations : University of Chicago, Illinois Institute of Technology

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Variable analysis

independent variables

Staining technique (hematoxylin-eosin or immunohistochemical/fluorescence staining)

dependent variables

Capillary density (mean number of vessels per square millimeter)

control variables

Monoclonal antibodies specific for von Willbrand factor (vWF), tropomyosin, and α-sarcomeric actinin in pigs

controls

No positive or negative controls were explicitly mentioned in the provided information.

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