H. pylori isolates were additionally confirmed using the 16S rRNA-based PCR method. Colonies were sub-cultured on Wilkins Chalgren anaerobe broth supplemented with the same materials declared above.16 (link),17 (link) Genomic DNA was then extracted using a DNA extraction kit (Thermo Fisher Scientific, St. Leon-Rot, Germany). Technique was performed rendering to the factory guidelines. Purity (A260/A280) and concentration of extracted DNA were then plaid (NanoDrop, Thermo Scientific, Waltham, MA) and the DNA quality was scrutinized by electrophoresis. PCR was accompanied using a PCR thermal cycler (Eppendorf Co., Hamburg, Germany) rendering to the described procedure.18 (link)
H. pylori 26695 was used as positive, while sterile PCR grade water (Thermo Fisher Scientific) was used as negative controls.
H. pylori 26695 was used as positive, while sterile PCR grade water (Thermo Fisher Scientific) was used as negative controls.
