The distal sciatic nerve stumps were harvested for nerve histomorphometry. Nerves were prepared as per established protocols.17 (link),18 (link) Nerve tissues were immersion fixed in 3% glutaraldehyde at 4°C and postfixed using 1% osmium tetroxide. Serial dehydration was performed following fixation using ethanol, and specimens were embedded using Araldite 502 and cut into semithin sections followed by staining with 1% toluidine blue dye and mounting onto glass slides for imaging. Leco IA32 Image Analysis System was used for quantification of nerve samples. This setup was used to calculate the total fascicle area of the nerve specimen. To calculate the total axons, myelin width, percentage fibers, and axonal density, 5 randomly selected high-magnification images (1000×) per sample were used. Data comparison was performed using Newman-Keul’s post hoc test.
Protocol detail
Nerve Histomorphometry Quantification Protocol
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Araldite
Axonal
Dehydration
Ethanol
Glutaraldehyde
Immersion
Myelin
Nerve
Nerve tissues
Osmium tetroxide
Sciatic nerve
Stumps
Toluidine blue
Corresponding Organization : The University of Texas at Arlington
Other organizations : Medical College of Wisconsin, Nerve Centre
Top 5 similar protocols
Variable analysis
independent variables
- None explicitly mentioned
- Total fascicle area of the nerve specimen
- Total axons
- Myelin width
- Percentage fibers
- Axonal density
- Nerve tissues were immersion fixed in 3% glutaraldehyde at 4°C
- Specimens were embedded using Araldite 502 and cut into semithin sections
- Sections were stained with 1% toluidine blue dye and mounted onto glass slides for imaging
- Positive control: Not specified
- Negative control: Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!