Quantification of VAR2CSA-specific IgG by ELISA
Corresponding Organization : Rigshospitalet
Other organizations : Sanquin, University of Copenhagen, Noguchi Memorial Institute for Medical Research, University of Ghana, University of Cape Coast, Mère et Enfant en Milieu Tropical, Université Paris Cité, Institut de Recherche pour le Développement, Netherlands Metabolomics Centre, Leiden University Medical Center, Radboud University Nijmegen, Radboud University Medical Center, Université d'Abomey-Calavi, University of Amsterdam, Amsterdam University Medical Centers
Variable analysis
- Monoclonal antibody concentration (0.08–10 μg/mL)
- Plasma sample dilution (1:400)
- Levels of VAR2CSA-specific IgG (measured in arbitrary units)
- Coating of 96-well plates with full-length VAR2CSA (100 ng/well in PBS)
- Washing of plates
- Addition of horseradish peroxidase-conjugated rabbit anti-human IgG (1:3,000)
- Detection of bound antibodies using TMB PLUS2 and 0.2 M H2SO4
- Positive control: Not explicitly mentioned
- Negative control: Blank (OD_BLANK)
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