Protein Extraction and Western Blotting

Protein extraction and Western Blotting were performed as described [51 (link)]. Used antibodies were a rabbit anti-Lcn2 antibody (1:1000; abcam, Cambridge, United Kingdom), a mouse anti-TFR1 antibody (1:1000; Sigma Aldrich), a rabbit anti-ferritin antibody (1:500; Sigma), a rabbit anti-Ho1 antibody (1:1000; abcam), a rabbit anti-Irp2 antibody (1:1000; Novusbio, Littleton, CO, USA) and a rabbit actin antibody (1:500; Sigma Aldrich), and appropriate HRP-conjugated secondary antibodies (1:2000, anti-rabbit; 1:4000, anti-mouse; Dako, Glostrup, Denmark). For quantification, densitometry data were acquired on a ChemiDoc Touch Imaging System (Bio-Rad, Hercules, CA, USA) and analyzed with Image Lab 5.2.1. (Bio-Rad).

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Haschka D., Grander M., Eibensteiner J., Dichtl S., Koppelstätter S, & Weiss G. (2021). Nifedipine Potentiates Susceptibility of Salmonella Typhimurium to Different Classes of Antibiotics. Antibiotics, 10(10), 1200.

Publication 2021

rabbit anti-Lcn2 antibody mouse anti-TFR1 antibody rabbit anti-ferritin antibody rabbit anti-Ho1 antibody rabbit actin antibody ChemiDoc Touch Imaging System Image Lab 5.2.1.

Actin Anti antibody Antibodies Antibody Densitometry Ferritin Mouse Protein Rabbit Tfr1 Touch

Corresponding Organization : Universität Innsbruck

Top 5 similar protocols

Variable analysis

independent variables

Lcn2 antibody (1:1000; abcam, Cambridge, United Kingdom)
TFR1 antibody (1:1000; Sigma Aldrich)
Ferritin antibody (1:500; Sigma)
Ho1 antibody (1:1000; abcam)
Irp2 antibody (1:1000; Novusbio, Littleton, CO, USA)
Actin antibody (1:500; Sigma Aldrich)

dependent variables

Protein expression levels of Lcn2, TFR1, Ferritin, Ho1, and Irp2

control variables

Protein extraction and Western Blotting methods described in reference [51]
HRP-conjugated secondary antibodies (1:2000, anti-rabbit; 1:4000, anti-mouse; Dako, Glostrup, Denmark)
Densitometry data acquisition on a ChemiDoc Touch Imaging System (Bio-Rad, Hercules, CA, USA) and analysis with Image Lab 5.2.1. (Bio-Rad)

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