Cell migration was evaluated by Transwell assay using the 8 μm-pore Transwell migration chambers (Corning, USA). Briefly, transfected cells were seeded in the upper portion of a chamber with serum-free medium. Complete medium filled in the lower chamber as an attractant. At 48 h after incubation, the non-migrated cells in the upper chamber were removed by cotton swabs. The migrated cells were stained with 0.1% crystal violet (Beyotime, Nantong, China) for 30 min, and then photographed and counted under optical microscope (Olympus, Tokyo, Japan).19 (link)
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Transwell Assay for Cell Migration
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Corresponding Organization : Capital Medical University
Other organizations : Heze Municipal Hospital
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Variable analysis
independent variables
- Transfection of cells
- Cell migration
- Pore size of Transwell migration chambers (8 μm)
- Incubation time (48 h)
- Staining method (0.1% crystal violet for 30 min)
- Optical microscope (Olympus, Tokyo, Japan) for imaging and counting migrated cells
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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