Differential interference contrast (DIC), phase-contrast, and fluorescence microscopy analyses were performed using a Nikon Eclipse 80i microscope equipped with an X-Cite TURBO multiwavelength LED illumination system and an Andor Zyla 4.2 sCMOS camera, as described previously (24 (link)). Quantitative image analyses were performed using the Oufti and MicrobeJ software packages (63 (link), 64 (link)).
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