Quantitative Analysis of Pigmentation Genes

Total RNA was reverse transcribed into cDNA using the Quantitect Reverse Transcription kit (Qiagen). Quantitative PCR (qPCR) was performed in SYBR Green master mix (Qiagen) with an Applied Biosystems 7500 PCR and analyzed with the SDS software (Keenen et al., 2010 (link)) (Thermo Fisher). The mRNA levels of human pigmentation genes MITF, SOX10, TYR, TYRP1, DCT, and IRF4 were normalized to ACTβ. Corresponding murine mRNA levels of Mitf, Sox10, Tyr, Tyrp1, and Dct were normalized to Rpl7. Oligonucleotide primer sequences are listed in Table S1.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Basuroy T., Dreier M., Baum C., Blomquist T., Trumbly R., Filipp F.V, & de la Serna I.L. (2022). Epigenetic and pharmacological control of pigmentation via Bromodomain Protein 9 (BRD9). Pigment Cell & Melanoma Research, 36(1), 19-32.

Publication 2022

Quantitect Reverse Transcription kit SYBR Green master mix SDS software

Cdna Genes Human Irf4 Mitf Mrna Murine Oligonucleotide primer Pigmentation Reverse transcription Sox10 Sybr green Tyrp1

Corresponding Organization : University of Toledo

Other organizations : Helmholtz Zentrum München, Weihenstephan-Triesdorf University of Applied Sciences

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

MRNA levels of human pigmentation genes MITF, SOX10, TYR, TYRP1, DCT, and IRF4
Corresponding murine mRNA levels of Mitf, Sox10, Tyr, Tyrp1, and Dct

control variables

ACTβ (for human pigmentation genes)
Rpl7 (for murine pigmentation genes)

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!