SARS-CoV-2 Pseudovirus Infection Assay

HIV-1 backbone-based Pseudovirus (PsV) bearing wild or mutant SARS-CoV2 S protein was produced, as previously described [27 (link)]. Caco2 cells were used as target cells and were seeded in wells of a 96-well plate 48 h in advance. PsV in the presence or absence of each peptide at indicated concentrations was added into target cells. After 12 h, culture medium was refreshed and continuously cultured for an additional 48 h. Then, cells were lysed with lysis reagent (Promega) for relative light units (RLU) detection by using Luciferase Assay Kits.

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Xia S., Wang L., Jiao F., Yu X., Xu W., Huang Z., Li X., Wang Q., Zhu Y., Man Q., Jiang S, & Lu L. (2023). SARS-CoV-2 Omicron subvariants exhibit distinct fusogenicity, but similar sensitivity, to pan-CoV fusion inhibitors. Emerging Microbes & Infections, 12(1), 2178241.

Publication 2023

lysis reagent Luciferase Assay Kits

Assay Backbone Caco2 cells Cells Culture medium Hiv 1 Light Luciferase Mutant protein Peptide Promega Sars

Corresponding Organization : Tongji University

Other organizations : Chinese Academy of Sciences, Institute of Biophysics

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Variable analysis

independent variables

Presence or absence of each peptide
Concentration of each peptide

dependent variables

Relative light units (RLU) detection

control variables

Caco2 cells as target cells
Seeding of Caco2 cells 48 h in advance
Culture medium refreshed after 12 h and continuously cultured for an additional 48 h

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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