Genetic Tracing of Adult Stem Cells

Lgr5−EGFP-IRES-CreER (JAX mice strain 008875), Bmi1-CreER (JAX strain 010531), and R26-CAG-LSL-tdTomato (JAX mice strain 007914) mice were obtained from the Jackson Laboratory. Hopx-CreER (JAX strain 017606) mice were generated at the University of Pennsylvania. Mice were maintained on a C57/BL6N background. Axin2-CreERT2-tdTomato mice were generated by insertion of a CreERT2/tdTomato fusion cDNA downstream of the first ATG of the mouse Axin2 gene using homologous recombination in mouse ESCs, described in detail in (Choi et al., 2013 (link)). For CreERT2 induction experiments, mice received 1–3 daily intraperitoneal injections of 100 μl Tam (10 mg/ml in corn oil; Sigma; T5648). All mouse protocols were approved by the Institutional Animal Care and Use Committee at the University of Pennsylvania under protocol 803415 to Dr. Lengner.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Li N., Yousefi M., Nakauka-Ddamba A., Tobias J.W., Jensen S.T., Morrisey E.E, & Lengner C.J. (2016). Heterogeneity in readouts of canonical Wnt pathway activity within intestinal crypts. Developmental dynamics : an official publication of the American Association of Anatomists, 245(8), 822-833.

Publication 2016

Lgr5−EGFP-IRES-CreER Bmi1-CreER

At protocol Axin2 Bmi1 Cdna Corn oil Escs Gene Homologous recombination Institutional animal care and use committee Intraperitoneal injections Ires Mouse Strain Tdtomato

Corresponding Organization : California Institute for Regenerative Medicine

Top 5 similar protocols

Variable analysis

independent variables

Lgr5−EGFP-IRES-CreER (JAX mice strain 008875)
Bmi1-CreER (JAX strain 010531)
R26-CAG-LSL-tdTomato (JAX mice strain 007914)
Hopx-CreER (JAX strain 017606)
Axin2-CreERT2-tdTomato

dependent variables

Not explicitly mentioned

control variables

Mice were maintained on a C57/BL6N background

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!