Automated Capillary Immunoassay for Protein Analysis

ProteinSimple® capillary-based immunoassay (The Jesss system, ProteinSimple®, San Jose, CA) was performed as previously reported 69 (link). The technology is an automated capillary size separation and nanoimmunoassay system that incorporates and automates the entire protein separation and detection process using homemade antigens. According to the manufacturer's protocol, primary antibodies were diluted targeting the following proteins: PDGFRα (1:50, Cell Signaling Technology), MBP (1:50, Abcam), Olig2 (1:50, R&D Systems). Antibody targets were detected with HRP-conjugated secondary antibodies. Digital images were analyzed using Compass for SW software (V6.1.0, Protein Simple) and quantified data of detected proteins were reported.