Isolation and Expansion of Human MSCs

MSCs were isolated from the stromal-vascular fraction of subcutaneous adipose tissues of 36 donors (age 42.17 ± 2.07 years; BMI 29.36 ± 1.01 kg/m², for details, see Tables S1 and S3) using enzymatic digestion [8 (link)]. These cells were CD45-/CD73+/CD105+/CD90+/NG2+/PDGFRβ+ [9 (link)]. All donors gave their informed consent. The study was conducted according to the guidelines of the Declaration of Helsinki and human primary cell collection was approved by the Local Ethic Committees of Burdenko Main Military Clinical Hospital (Moscow, Russia) and the Medical Research and Education Center of Lomonosov Moscow State University (IRB00010587, Moscow, Russia) approved the study protocol (#160, 22 July 2019 and #4, 4 June 2018, respectively). Donors medication presented in Table S1. Primary MSCs were cultured in AdvanceSTEM Mesenchymal Stem Cell Media containing a 10% AdvanceSTEM Supplement (HyClone, Cytiva, Marlborough, MA, USA), 1% antibiotic–antimycotic solution (HyClone, Cytiva, Marlborough, MA, USA) at 37 °C in a 5% CO₂ incubator (Binder, Tuttlingen, Germany, CB210). Cells were passaged at 70–80% confluency using Versen solution (Paneco, Moscow, Russia) and HyQTase solution (HyClone, Cytiva, Marlborough, MA, USA).