Quantification of Amaranthus Extracts

AMK extracts were obtained from root crude drug as previously reported (Wang et al. 2016 (link)). Briefly, AMK was percolated with 95% ethanol, 15.87 g of crude drug was concentrated to 1 g of AMK extracts. AMK extracts were analysed using high-performance liquid chromatography (HPLC) on a Waters 600E system (Waters, Milford, MA, USA) equipped with ultraviolet (UV) absorbance, refractive index detectors, and a C₁₈ column (Kromasil, Sweden). 1.09% AA I was detected in the AMK extracts.

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Wang L., Li C., Tian J., Liu J., Zhao Y., Yi Y., Zhang Y., Han J., Pan C., Liu S., Deng N., Xian Z., Li G., Zhang X, & Liang A. (2020). Genome-wide transcriptional analysis of Aristolochia manshuriensis induced gastric carcinoma. Pharmaceutical Biology, 58(1), 98-106.

Publication 2020

Waters 600E system

Drug Ethanol High performance liquid chromatography Root

Corresponding Organization : Chinese Academy of Medical Sciences & Peking Union Medical College

Other organizations : Shenzhen Blood Center

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Variable analysis

independent variables

Extraction of AMK from root crude drug

dependent variables

Concentration of AMK extracts
Percentage of AA I in AMK extracts

control variables

95% ethanol used for percolation
HPLC system with UV absorbance and refractive index detectors
C18 column for HPLC analysis

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