SPECT Imaging of Apoptotic C6 Cells

For SPECT imaging in vitro, the apoptotic and normal C6 cells cultured under the same conditions described above were incubated with the ^99mTc-duramycin-Au DENPs or ^99mTc-Au DENPs at different radiodoses (25, 50, 100, 200, and 400 μCi/mL) for 4 h. Then, the cells were treated under the above conditions and scanned according to our previous work (Li et al., 2016 (link)). Similarly, apoptotic and normal C6 cells were incubated with fresh medium containing PBS, duramycin-Au DENPs, or Au DENPs at different Au concentrations (20, 40, 60, 80, and 100 μM) for 3 h. After appropriate treatment according to our previous work (Zhou et al., 2018 (link)), the cells were imaged by a GE Discovery STE PET/CT system with a tube voltage of 100 kV, an electrical current of 220 mA, and a slice thickness of 1.25 mm.