Evaluating Cell Viability and Apoptosis

Cell viability was determined by trypan blue exclusion assay (Invitrogen) or by flow cytometry of cells stained with the fluorescent DNA intercalator TO-PRO 3 (Invitrogen). The colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich) assay was performed as previously described^{19 (link)}. For cell cycle and death analyses, flow cytometry of cells stained with annexin V and propidium iodide (Invitrogen) was performed using the Guava EasyCyte flowcytometry system (MilliporeSigma, Billerica, MA, USA), as previously described^{47 (link)}. Data were analyzed by FCS EXPRESS software (De Novo Software, Los Angeles, CA, USA).

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Wu P.K., Hong S.K., Starenki D., Oshima K., Shao H., Gestwicki J.E., Tsai S, & Park J.I. (2020). Mortalin/HSPA9 targeting selectively induces KRAS tumor cell death by perturbing mitochondrial membrane permeability. Oncogene, 39(21), 4257-4270.

Publication 2020

trypan blue exclusion assay MTT annexin V and propidium iodide Guava EasyCyte flowcytometry system FCS EXPRESS software

Annexin v Assay Bromide Cell cycle Cell viability Cells Colorimetric Dna intercalator Flowcytometry Guava Propidium iodide Trypan blue

Corresponding Organization :

Other organizations : Medical College of Wisconsin, Johns Hopkins University, Johns Hopkins Medicine, University of California, San Francisco

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Variable analysis

independent variables

Cell treatment

dependent variables

Cell viability (measured by trypan blue exclusion assay or flow cytometry of TO-PRO 3-stained cells)
Cell cycle and death (measured by flow cytometry of annexin V and propidium iodide-stained cells)
Cell metabolic activity (measured by MTT assay)

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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