Affinity-purified antibodies for CLIC protein detection

AP255 and AP1089, the affinity-purified rabbit polyclonal antibodies to CLIC4 and CLIC1, respectively, have been previously described [17 (link),32 (link)]. Commercial antibodies were as follows: Goat polyclonal antibody to mouse albumin, Bethyl Labs (Montgomery TX) #A90-134; rat monoclonal antibody to CD31 clone MEC13.3, Pharmingen (San Jose, CA) #550274; mouse monoclonal antibody to PCNA, Cell Signaling Technology (Danvers, MA) #2586; rabbit monoclonal antibody to Smad2/3, Cell Signaling Technology #8685; rabbit monoclonal antibody to phospho-Smad2/3, Sigma-Aldrich #SAB4504208; mouse monoclonal antibody to GAPDH, Santa Cruz Biotechnology (Santa Cruz, CA) #SC-32233; goat polyclonal antibody to CLIC5, Santa Cruz Biotechnology #SC-65041 Alexa Fluor 488 anti rat IgG, Life Technologies (Grand Island, NY); Cy5 goat anti rabbit IgG, Jackson Labs (Bar Harbor, ME) #111-175-144; FITC-lectin from Lotus tetragonolobus (LTA), Vector Labs (Burlingame CA) #FL-1321; Alexa Fluor 594-isolectin B4 from Griffonia simpicifolia (IB4), Life Technologies, #I21413; HRP-conjugated secondary antibodies, Thermo Scientific Pierce.

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Edwards J.C., Bruno J., Key P, & Cheng Y.W. (2014). Absence of chloride intracellular channel 4 (CLIC4) predisposes to acute kidney injury but has minimal impact on recovery. BMC Nephrology, 15, 54.

Publication 2014

mouse monoclonal antibody to GAPDH Alexa Fluor 488 anti rat IgG Cy5 goat anti rabbit IgG

Albumin Alexa 594 Alexa fluor 488 Anti igg Antibodies Antibodies affinity Antibody Clic4 Clone Fitc Gapdh Goat Griffonia Isolectin Lotus tetragonolobus lectin Monoclonal antibody Mouse Pcna Rabbit Smad2 Vector

Corresponding Organization :

Other organizations : Saint Louis University, University of North Carolina at Chapel Hill

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Variable analysis

independent variables

AP255 and AP1089, the affinity-purified rabbit polyclonal antibodies to CLIC4 and CLIC1, respectively

dependent variables

Not explicitly mentioned

control variables

Goat polyclonal antibody to mouse albumin, Bethyl Labs (Montgomery TX) #A90-134
Rat monoclonal antibody to CD31 clone MEC13.3, Pharmingen (San Jose, CA) #550274
Mouse monoclonal antibody to PCNA, Cell Signaling Technology (Danvers, MA) #2586
Rabbit monoclonal antibody to Smad2/3, Cell Signaling Technology #8685
Rabbit monoclonal antibody to phospho-Smad2/3, Sigma-Aldrich #SAB4504208
Mouse monoclonal antibody to GAPDH, Santa Cruz Biotechnology (Santa Cruz, CA) #SC-32233
Goat polyclonal antibody to CLIC5, Santa Cruz Biotechnology #SC-65041
Alexa Fluor 488 anti rat IgG, Life Technologies (Grand Island, NY)
Cy5 goat anti rabbit IgG, Jackson Labs (Bar Harbor, ME) #111-175-144
FITC-lectin from Lotus tetragonolobus (LTA), Vector Labs (Burlingame CA) #FL-1321
Alexa Fluor 594-isolectin B4 from Griffonia simpicifolia (IB4), Life Technologies, #I21413
HRP-conjugated secondary antibodies, Thermo Scientific Pierce

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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