Quantification of Liver Enzymes and Cytokine

Plasma levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined using a Transaminase CII kit (Wako Pure Chemical Industries, Osaka, Japan), as previously described [24 (link)–26 (link)]. Plasma levels of tumor necrosis factor alpha (TNFα) were measured using a commercially available ELISA kit (eBioscience, San Diego, CA, USA), as previously described [22 (link)]. For relative quantification, calibration curves were prepared using standard solutions.

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Yoshioka H., Nonogaki T., Fukaya S., Ichimaru Y., Nagatsu A., Yoshikawa M., Fujii H, & Nakao M. (2018). Sasa veitchii extract protects against carbon tetrachloride-induced hepatic fibrosis in mice. Environmental Health and Preventive Medicine, 23, 49.

Publication 2018

Transaminase CII kit

Alanine aminotransferase Aspartate aminotransferase Elisa Plasma Tnfα Transaminase

Corresponding Organization :

Other organizations : Kinjo Gakuin University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Plasma levels of alanine aminotransferase (ALT)
Plasma levels of aspartate aminotransferase (AST)
Plasma levels of tumor necrosis factor alpha (TNFα)

control variables

None explicitly mentioned

controls

Calibration curves were prepared using standard solutions for relative quantification.

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