Procedures were as described previously (Li et al., 2020 (link)). The primary antibody information is listed below: SMAD4 (Abcam, ab110175), β-actin (Abcam, ab6276), cyclin-dependent kinase 4 (CDK4) (Abcam, ab193968), cyclin D1 (Abcam, ab40754), Bcl-2 (Abcam, ab182858), Bax (Abcam, ab263897), and Caspase-3 (Abcam, ab32351).
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