Ristocetin was purchased from MP Biomedicals. Most recombinant VWF fragments and type 2B mutants were expressed from baby hamster kidney cells as described19 (link),27 (link). Plasma-derived VWF was reconstituted in phosphate-buffered saline (PBS) from outdated lyophilized Humate-P (CSL-Behring). Monoclonal antibodies 6G1, CR1, and 11A8 have been described19 (link),47 (link). ARC117255 (link) was synthesized by Integrated DNA Technologies. Mammalian cells were maintained in DMEM/F12 culture media (ThermoFisher), with 10% Foundation-B fetal bovine serum (Gemini Bio-Products, Sacramento, CA), and 1% penicillin/streptomycin (ThermoFisher). Bovine serum albumin, fraction V was purchased from Fisher Scientific. Tobacco etch virus (TEV) protease was produced with plasmid pD2087 in BL21pRIL cells and purified as described79 (link). The Human GPIb-IX complex was purified from outdated and deidentified leuko-reduced apheresis-derived platelets as described80 (link).
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