DNA Damage Response Signaling Immunoblotting

Immunoblot analysis was performed as described in detail previously [21 (link),22 (link)]. Primary antibodies against ATM (Bethyl Laboratories, Montgomery, TX, USA), phospho-ATM Ser1981 (Cell Signaling Technologies, Danvers, NJ, USA), ATR (Bethyl Laboratories), phospho-ATR Ser428 (Cell Signaling Technologies), p53 (Cell Signaling Technologies), phospho-p53 Ser15 (Cell Signaling Technologies), SMC1 (Bethyl Laboratories), phospho-SMC1 Ser966 (Bethyl Laboratories), Chk1 (G-4; Santa Cruz Biotechnology, Santa Cruz, CA, USA), phospho-Chk1 Ser317 (Bethyl Laboratories), phospho-Chk1 Ser345 (Cell Signaling Technologies), Chk2 (H-300; Santa Cruz), phospho-Chk2 Thr68 (Cell Signaling Technologies), Wip1 (PPM1D; Bethyl Laboratories), Cdc25A (F-6; Santa Cruz) and GAPDH (Cell Signaling Technologies) were used for immunoblots.

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Tatewaki N., Konishi T., Nakajima Y., Nishida M., Saito M., Eitsuka T., Sakamaki T., Ikekawa N, & Nishida H. (2016). Squalene Inhibits ATM-Dependent Signaling in γIR-Induced DNA Damage Response through Induction of Wip1 Phosphatase. PLoS ONE, 11(1), e0147570.

Publication 2016

phospho-ATM Ser1981 phospho-ATR Ser428 phospho-p53 Ser15 Chk1 (G-4; phospho-Chk1 Ser317 phospho-Chk1 Ser345 phospho-Chk2 Thr68 Cdc25A (F-6; GAPDH

Antibodies Cdc25a Gapdh Immunoblots Ppm1d

Corresponding Organization : Niigata Agricultural Research Institute

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Variable analysis

independent variables

Primary antibodies against ATM, phospho-ATM Ser1981, ATR, phospho-ATR Ser428, p53, phospho-p53 Ser15, SMC1, phospho-SMC1 Ser966, Chk1, phospho-Chk1 Ser317, phospho-Chk1 Ser345, Chk2, phospho-Chk2 Thr68, Wip1, Cdc25A

dependent variables

Immunoblot analysis

control variables

GAPDH

controls

Positive controls not explicitly mentioned.
Negative controls not explicitly mentioned.

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