IL-2/JES6-1 immunocomplexes were prepared by pre-incubating recombinant IL-2 (PeproTech) with anti-IL-2 mAb (JES6-1; Bioxcell) at a 2:1 cytokine:antibody molar ratio for 30 min at room temperature (23 (link), 65 (link)). Mice were injected with anti-IL-5 antibodies (200 μg) and/or IL-2/JES6-1 immunocomplexes (1 mg every 2 days) by i.p injection.
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