Isolation and Analysis of PBMCs

Human PBMCs were isolated from heparinized venous blood using Lymphoprep (1114545; Axis-shield) in accordance with the manufacturer’s instructions. Total RNA from PBMCs was extracted using TRIzol reagent (15596026; Invitrogen) in accordance with the manufacturer’s instructions, followed by RNA quantification and assessment using QIAxpert (Qiagen). Nanostring nCounter Human Immunology gene expression assays for PBMCs were performed at PhileKorea Technology (Daejeon, South Korea), and the data were analyzed by nSolver version 4.0 (NanoString Technologies) as described previously.^{32 (link)}

Free full text: Click here

Kim Y.J., Lee J.Y., Lee J.J., Jeon S.M., Silwal P., Kim I.S., Kim H.J., Park C.R., Chung C., Han J.E., Choi J.W., Tak E.J., Yoo J.H., Jeong S.W., Kim D.Y., Ketphan W., Kim S.Y., Jhun B.W., Whang J., Kim J.M., Eoh H., Bae J.W, & Jo E.K. (2022). Arginine-mediated gut microbiome remodeling promotes host pulmonary immune defense against nontuberculous mycobacterial infection. Gut Microbes, 14(1), 2073132.

Publication 2022

Lymphoprep TRIzol reagent QIAxpert nSolver version 4.0

Assays Axis Gene expression Human Lymphoprep Trizol Venous blood

Corresponding Organization : University of Southern California

Other organizations : Samsung Medical Center, Sungkyunkwan University

Top 5 similar protocols

Variable analysis

independent variables

Isolation of human PBMCs from heparinized venous blood using Lymphoprep

dependent variables

Total RNA from PBMCs extracted using TRIzol reagent
RNA quantification and assessment using QIAxpert
Nanostring nCounter Human Immunology gene expression assays for PBMCs

control variables

Manufacturer's instructions for Lymphoprep and TRIzol reagent were followed

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!