Synthesis and Characterization of ISC-4 for Leukemia Research

ISC-4 was synthesized using our previously described method at Penn State College of Medicine Organic Synthesis Core (16 (link)). Purity of ISC-4 (≥97%) was quantified by analytical high performance liquid chromatography (HPLC) analysis. Cytarabine (AraC, NDC 61703-305-38), Azacitidine (Aza, NDC 63323-771-39), and Daunorubicin (DNR, NDC 0703-5233-11) were purchased from the Penn State Hershey Medical Center Pharmacy. AraC was stored at room temperature, DNR at 4°C, and Azacitidine at −80°C. Venetoclax was purchased from ChemieTek (Indianapolis). ISC-4, Venetoclax and GDC-0941 were reconstituted in DMSO and aliqouts were stored at −20°C. GDC-0941 (catalog # S1065) was purchased from Selleck Chemicals and reconstituted in DMSO. Aliquots were stored in −80°C.

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Annageldiyev C., Tan S.F., Thakur S., Dhanyamraju P.K., Ramisetti S.R., Bhadauria P., Schick J., Zeng Z., Sharma V., Dunton W., Dovat S., Desai D., Zheng H., Feith D.J., Loughran TP J.r., Amin S., Sharma A.K., Claxton D, & Sharma A. (2020). The PI3K/AKT Pathway Inhibitor ISC-4 Induces Apoptosis and Inhibits Growth of Leukemia in Preclinical Models of Acute Myeloid Leukemia. Frontiers in Oncology, 10, 393.

Publication 2020

Venetoclax GDC-0941

Arac Azacitidine Cytarabine Daunorubicin Dmso Gdc 0941 High performance liquid chromatography Medicine Organic synthesis method Venetoclax

Corresponding Organization : Penn State Milton S. Hershey Medical Center

Other organizations : University of Virginia

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Variable analysis

independent variables

Synthesis method of ISC-4 at Penn State College of Medicine Organic Synthesis Core

dependent variables

Purity of ISC-4 (≥97%) quantified by analytical high performance liquid chromatography (HPLC) analysis

control variables

Storage conditions for compounds: AraC at room temperature, DNR at 4°C, Azacitidine at −80°C, ISC-4, venetoclax, GDC-0941 at −20°C or −80°C

positive controls

Cytarabine (AraC, NDC 61703-305-38)
Azacitidine (Aza, NDC 63323-771-39)
Daunorubicin (DNR, NDC 0703-5233-11)

negative controls

Venetoclax purchased from ChemieTek (Indianapolis)
GDC-0941 (catalog # S1065) purchased from Selleck Chemicals

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