CSCs of OVCAR-3 and OVCAR-3/DDP (CSCs/DDP) were treated with 0, 10, 20, 40, and 80 µM of lumiflavin and 0, 40, 80, 160, 320 and 640 µM of DDP for 72 h. Surviving CSCs/DDP were compared with the CSCs combining cell viability/proliferation assays using the CCK-8 assay kit (Dojindo Laboratories, Japan) according to the manufacturer’s instruction. The sensitivity of these paired cell lines is usually determined by exposing them to a range of drug concentrations and then assessing cell viability. The IC50 (drug concentration causing 50% growth inhibition) for these paired cell lines can be used to determine the increase in resistance known as the resistance index by the following equation (21 (link)):
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