As an EPR sample, we prepared
a Copper II ions (a typical reference EPR sample already used in with
NV centers35 (link)). We prepared a nitric acid
solution by diluting concentrated nitric acid (90%, Sigma-Aldrich,
695041) in Milli-Q water. The sample itself was prepared by dissolving
copper sulfate powder (Merck, 2790) in nitric acid solution to favor
the formation of copper II hexaaqua Cu(H2O)6 complex ions,
which have a well-known EPR spectrum.36 (link) The final concentration of the solution is approximately 1 μm.
The sample was pumped through a Y shaped microfluidic channel (0.1
mm width, 0.5 mm thick) on a PDMS chip with a syringe.
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