Genotyping Candida Species by Microsatellites

Isolates were genotyped by species-specific microsatellite markers. Six markers were used for C. albicans: CDC3, EF3, HIS3 (Botterel et al., 2001 (link); Sabino et al., 2010 (link)), CAI, CAIII, and CAVI (Sampaio et al., 2005 (link)). Markers used for C. tropicalis were Ctrm1, Ctrm10, Ctrm12, Ctrm21, Ctrm24, and Ctrm28 (Wu et al., 2014 (link)), and CP1, CP4a, CP6, and B were used for C. parapsilosis (Sampaio et al., 2003 (link); Vaz et al., 2011 (link)).
Capillary electrophoresis using the ABI 3130xl analyser (Applied Biosystems-Life Technologies Corporation, Carlsbad, California, USA) and the GeneScan ROX 500 bp marker (Applied Biosystems-Life Technologies Corporation, Carlsbad, California, USA) was performed on the PCR products. Electropherograms were analyzed with the GeneMapper® v.4.0 software (Applied Biosystems-Life Technologies Corporation, California). A control strain from each species was used in each run to ensure size accuracy and avoid run-to-run variations. The number of base pairs determined the size of alleles in each locus.

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Guinea J., Arendrup M.C., Cantón R., Cantón E., García-Rodríguez J., Gómez A., de la Pedrosa E.G., Hare R.K., Orden B., Sanguinetti M., Pemán J., Posteraro B., Ruiz-Gaitán A., Parisi G., Da Matta D.A., Colombo A.L., Sánchez-Carrillo C., Reigadas E., Muñoz P, & Escribano P. (2020). Genotyping Reveals High Clonal Diversity and Widespread Genotypes of Candida Causing Candidemia at Distant Geographical Areas. Frontiers in Cellular and Infection Microbiology, 10, 166.

Publication 2020

ABI 3130xl analyser GeneMapper® v.4.0 software

Alleles Capillary electrophoresis Cavi Microsatellite markers Strain

Corresponding Organization : Hospital General Universitario Gregorio Marañón

Other organizations : Rigshospitalet, Statens Serum Institut, University of Copenhagen, Instituto Ramón y Cajal de Investigación Sanitaria, Red Espanola de Investigacion en Patologia Infecciosa, Instituto de Salud Carlos III, Instituto de Investigación Sanitaria La Fe, Universitat de València, Hospital Universitario La Paz, Hospital Universitario Puerta de Hierro Majadahonda, Agostino Gemelli University Polyclinic, Istituti di Ricovero e Cura a Carattere Scientifico, Hospital Universitari i Politècnic La Fe, Azienda Ospedaliera San Camillo-Forlanini, Universidade Federal de São Paulo

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Variable analysis

independent variables

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control variables

Control strain from each species was used in each run to ensure size accuracy and avoid run-to-run variations

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