The DPPH
assay was carried out to investigate the suppression of free radical
properties of NAR–CM–CS/SA–NPs and NAR–suspension
as per modified procedure by Gabriele et al.62 (link) For the preparation of stock solution, 10 mg of NAR–CM–CS/SA–NPs,
CIP + NAR–CM–CS/SA–NPs, and NAR–suspension
was transferred into a volumetric flask, leveled stock solution formulation,
and drug suspension. Dilutions of each stock solution were prepared
ranging from 10 to 640 μM. The DPPH reagent of 0.03% w/v was
prepared in ethanol and 100 μL was mixed with 1 mL of sample
in the specified range. The mixer was vortexed and this complete set
of samples was kept in a dark place at ambient temperature for 1 h.
Thereafter, the complete samples were examined for color change owing
to the reaction between the DPPH reagent and sample, i.e., violet
to colorless. The maximum change in violet color to colorless suggests
the better antioxidant capacity of the sample. Ethanol was used as
a blank. Now, the complete samples were estimated by UV–visible
spectroscopy at a specific wavelength of 517 nm using butylated hydroxytoluene
(BHT) as the reference standard for comparing the antioxidant capacity.
The antioxidant capacity of Naringin as percentage inhibition was
plotted versus concentration. The IC50 was determined by data interpolation
and compared with standard. The antioxidant capacity was determined
using a formula. where A0 is blank sample absorbance; A sample
absorbance.
assay was carried out to investigate the suppression of free radical
properties of NAR–CM–CS/SA–NPs and NAR–suspension
as per modified procedure by Gabriele et al.62 (link) For the preparation of stock solution, 10 mg of NAR–CM–CS/SA–NPs,
CIP + NAR–CM–CS/SA–NPs, and NAR–suspension
was transferred into a volumetric flask, leveled stock solution formulation,
and drug suspension. Dilutions of each stock solution were prepared
ranging from 10 to 640 μM. The DPPH reagent of 0.03% w/v was
prepared in ethanol and 100 μL was mixed with 1 mL of sample
in the specified range. The mixer was vortexed and this complete set
of samples was kept in a dark place at ambient temperature for 1 h.
Thereafter, the complete samples were examined for color change owing
to the reaction between the DPPH reagent and sample, i.e., violet
to colorless. The maximum change in violet color to colorless suggests
the better antioxidant capacity of the sample. Ethanol was used as
a blank. Now, the complete samples were estimated by UV–visible
spectroscopy at a specific wavelength of 517 nm using butylated hydroxytoluene
(BHT) as the reference standard for comparing the antioxidant capacity.
The antioxidant capacity of Naringin as percentage inhibition was
plotted versus concentration. The IC50 was determined by data interpolation
and compared with standard. The antioxidant capacity was determined
using a formula. where A0 is blank sample absorbance; A sample
absorbance.
