The majority of ICRs are methylated on the maternally inherited allele. During oogenesis, DNA methyltransferase 3-like (Dnmt3l) is required for the establishment of these methylation imprints.21 (link)
Dnmt3l-deficient dams were crossed with wildtype sires to obtain Dnmt3l−/+ embryos that lack maternal methylation imprints.21 (link) Total RNA from eight and six (two distinct litters) 8.5 dpc Dnmt3l−/+ embryos and their visceral yolk sacs was pooled and gene expression assayed using Affymetrix 430 2.0 microarrays, constituting two biologically independent replicate experiments. A single wildtype sample derived from six pooled 8.5 dpc embryos including their yolk sacs was assayed to provide a baseline of expression. The microarray hybridisations were performed according to the standard Affymetrix protocols, as described in ref. 27 (link). The raw array data are available from the Gene Expression Omnibus (GEO) under accession number GSE8756.
Dnmt3l-deficient dams were crossed with wildtype sires to obtain Dnmt3l−/+ embryos that lack maternal methylation imprints.21 (link) Total RNA from eight and six (two distinct litters) 8.5 dpc Dnmt3l−/+ embryos and their visceral yolk sacs was pooled and gene expression assayed using Affymetrix 430 2.0 microarrays, constituting two biologically independent replicate experiments. A single wildtype sample derived from six pooled 8.5 dpc embryos including their yolk sacs was assayed to provide a baseline of expression. The microarray hybridisations were performed according to the standard Affymetrix protocols, as described in ref. 27 (link). The raw array data are available from the Gene Expression Omnibus (GEO) under accession number GSE8756.
