Genetically Modified Mice for Immune Research

C57BL/6J (CD45.1⁺), C57BL/6J (CD45.2⁺), T-bet-ZsGreen^{35 (link)} (Taconic Biosciences), Rbpj^loxP/loxP^{50 (link)} (abbreviated to ΔRBPJ), Clec9a^Cre28 (abbreviated to C9a), Flt3l^−/− (Taconic Biosciences), Rosa26^LSL-tdTomato (The Jackson Laboratory) mice were bred at the Francis Crick Institute in specific pathogen-free conditions. Siglech^iCre mice^{22 (link)} (B6-Siglech^{tm1(iCre)Ciphe}) were generated by the Centre d’Immunophénomique (Marseille, France) and crossed to the Rosa26^LSL-RFP^{51 (link)} and Lyz2^eGFP^{42 (link)} strains. All genetically modified mouse lines were backcrossed to C57BL/6J; 6–12-week-old male and female mice were age-matched and sex-matched in all experiments.

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Minutti C.M., Piot C., Pereira da Costa M., Chakravarty P., Rogers N., Huerga Encabo H., Cardoso A., Loong J., Bessou G., Mionnet C., Langhorne J., Bonnet D., Dalod M., Tomasello E, & Reis e Sousa C. (2024). Distinct ontogenetic lineages dictate cDC2 heterogeneity. Nature Immunology, 25(3), 448-461.

Publication 2024

C57BL/6J Flt3l−/− Rosa26LSL-tdTomato

Corresponding Organization :

Other organizations : Centre d’Immunologie de Marseille-Luminy

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Variable analysis

independent variables

C57BL/6J (CD45.1+)
C57BL/6J (CD45.2+)
T-bet-ZsGreen35
Rbpjloxp/loxp (ΔRBPJ)
Clec9aCre28 (C9a)
Flt3l-/-
Rosa26LSL-tdTomato
Siglech iCre
Rosa26LSL-RFP
Lyz2eGFP

dependent variables

Not explicitly mentioned

control variables

Age-matched and sex-matched C57BL/6J mice
Mice were bred in specific pathogen-free conditions

positive controls

Not specified

negative controls

Not specified

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