Mptx2-/- Mice in DSS-Induced Colitis

We used 6-week-old Mptx2^−/− mice (female, n = 6; male, n = 10) and their wild-type (Wt) (female, n = 9; male, n = 6) littermates for dextran sulfate sodium (DSS)–induced colitis experiments. Mptx2^−/− (female, n = 8; male, n = 7) mice and Wt (female, n = 8; male, n = 8) mice were untreated as controls. Acute colitis was induced by administration of 2% DSS (36–50 kDa; MP Biomedicals, Solon, OH, USA) in drinking water for 7 days. We monitored changes in mouse body weight (BW) daily. To construct the DSS-induced colitis and recovery mouse model, C57BL/6 mice were induced by 3.5% DSS for 7 days and allowed to recover for 2 weeks (day 0, female, n = 8; male, n = 7), (day 1, female, n = 5; male, n = 5), (day 3, female, n = 5; male, n = 5), (day 7, female, n = 5; male, n = 5), (recovery 1 week, female, n = 5; male, n = 5), and (recovery 2 week, female, n = 6; male, n = 5).