Gene Expression Patterns in Human Airways

Gene expression patterns of ABCF1 in human airway epithelial cells was determined relative to markers for immune cells (CD34), ABC transporters of known function in airway epithelial cells (ABCC4, ABCC7), and junctions (CDH1) in a dataset containing samples from trachea, large airways (generation 2^nd-3^rd), and small airways (generation 10^th-12^th) from healthy subjects (GSE11906, Affymetrix Human Genome U133 Plus 2 microarray platform) (Raman et al., 2009 (link)). The following probesets were used to extract gene expression data: ABCF1 (200045_at), ABCC4 (203196_at), ABCC7 (CFTR; 205043_at), CDH1 (201131_s_at), and CD34 (209543_s_at). In cases where more than one probe corresponded to a given gene, the following hierarchy was used to select an individual probe for further use: perfect, unique matches (probes ending in _at or _a_at) were preferred over mismatch or non-unique probes (ending in _s_at or _x_at). GSE11906 included 17 trachea (age−42 +/– 7), 21 large airway (age−42 +/– 9), and 35 small airway samples.